Transcriptome sequencing based on high-throughput sequencing technology can not only quickly and comprehensively obtain the expression of mRNA and non coding RNA in specific tissues or cells in specific states, but also accurately analyze the SNP, indel, gene fusion and alternative splicing of transcripts through the measured sequence information. Transcriptome sequencing has been widely used in biological growth and development research, disease occurrence and development research, animal and plant stress response to the environment and so on.
High throughput sequencing of all mRNAs produced by tissues or cells of a species in a specific state. Quantitative and differential expression analysis of genes can be carried out for the sequencing of participating transcripts. In addition, rare transcripts can be found, and gene structures such as variable shear sites and gene fusion can be analyzed for basic research, clinical diagnosis It is widely used in drug research and development and molecular breeding. UniGene is used as the reference sequence for subsequent analysis of nonparametric transcriptome sequencing, which plays an important role in the study of molecular mechanism and network regulation of nonparametric species.