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Higentec and Heilongjiang Academy of Agricultural Sciences have made new progress in japonica rice genome researchh

(Summary description)Recently, Higentec Biotechnology Co., Ltd. (Higentec Bio) cooperated with the Rice Research Institute of the Heilongjiang Academy of Agricultural Sciences to complete the whole genome resequencing of 239 japonica rice varieties from China, Japan and South Korea, and identified a total of 4.8 million 35,634 genes. remaining SNPs and PAVs.

Higentec and Heilongjiang Academy of Agricultural Sciences have made new progress in japonica rice genome researchh

(Summary description)Recently, Higentec Biotechnology Co., Ltd. (Higentec Bio) cooperated with the Rice Research Institute of the Heilongjiang Academy of Agricultural Sciences to complete the whole genome resequencing of 239 japonica rice varieties from China, Japan and South Korea, and identified a total of 4.8 million 35,634 genes. remaining SNPs and PAVs.

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Recently, Higentec Biotechnology Co., Ltd. (Higentec Bio) cooperated with the Rice Research Institute of the Heilongjiang Academy of Agricultural Sciences to complete the whole genome resequencing of 239 japonica rice varieties from China, Japan and South Korea, and identified a total of 4.8 million 35,634 genes. remaining SNPs and PAVs.

Compared with the main varieties in northeastern my country, the japonica rice varieties in Japan and South Korea are more closely related, and their diversity is lower than that of the Chinese varieties. The japonica pan genome was constructed, 35Mb non-redundant new sequences longer than 100bp were identified, and 1131 new full-length novel genes were predicted. Japonica rice varieties in Northeast my country have undergone strong artificial selection in the breeding process, especially the artificial selection of genes Hd1 and Hd3a at heading stage, to cultivate a new ecological type of early-maturing japonica rice that adapts to the climate of Northeast China. In-depth analysis of genes related to the resistance and quality of backup japonica rice in my country, such as Pi2, Pi3/5, Piz, Ptr, Pita, Xa21, Hd1, SCM2, SCM3, Cold1, pms3, Wx, badh2, etc. Japonica rice varieties have favorable genotypes of bacterial blight resistance gene Xa21 and cold tolerance gene Cold1. Among them, Longjing 31 and Longjing 46 are the cultivars with the largest planting area in my country, both with favorable alleles of SCM3 (lodging resistance). ), but Longjing 31 carries favorable alleles of Pita and Ptr, making it more resistant to rice blast than Longjing 46. Genome comparison revealed that more than 20 Longjing rice varieties were directly or indirectly derived from a Japanese introduced variety, Kongyu 131, and 95 Northeast japonica varieties had a genome similarity of more than 67% with Kongyu 131. Strong selection signals were detected in the genomic regions of chromosomes 2, 3, 6, 8, and 12 in the Longjing series. ), chalk5, ALK, Hd1, Ehd1, Hd6, Hd3a and other genes in-depth analysis found that the growth stage genes Hd1, Hd3a and Hd17 were artificially selected during the breeding process, which was completely consistent with the selection of early-maturing japonica varieties to adapt to the northern climate.

Author

The research results were published online in Genomics on July 6, 2021. The Rice Research Institute of Heilongjiang Academy of Agricultural Sciences provided the japonica rice samples for the study, and Higentec Biosciences carried out whole genome resequencing and in-depth data analysis. Liu Chuanxue, Peng Pei, Li Weiguo and Ye Changrong of Higentec Biotechnology Institute of Heilongjiang Academy of Agricultural Sciences are the co-first authors, and Pan Guojun of the Rice Institute of Heilongjiang Academy of Agricultural Sciences, Tian Glacier and Xiao Jinhua of Higentec Biological Sciences are co-corresponding authors.

2021-07-08

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