On September 22, 2020, nature communications magazine published online a research paper from yuan dingyang team of Hunan Hybrid Rice Research Center, Yuan Longping academician group and Li Dong of Huazhi biology, entitled "resequencing of 1143 indica rice access requests important genetic variations and Different Heterosis patterns". In this study, 1143 indica parental materials in hybrid rice breeding were re sequenced, which revealed that three-line and two-line Strong Heterosis Hybrid Rice Combinations had different heterosis control gene loci. This study provides a new method for discovering heterosis loci.

Firstly, by integrating population structure analysis and phylogenetic tree analysis, it is found that the three-line strong dominant combinations are mainly between indica rice subgroup I and indica rice subgroup II, while the two-line strong dominant combinations are mainly within indica rice subgroup II, and the mitochondrial genetic distance of the three-line strong dominant combinations is significantly greater than all possible combinations and two-line strong dominant combinations, The importance of mitochondrial genomic differences among parents of three-line hybrid rice was confirmed from the perspective of molecular genetics. In addition, the study also analyzed the three-line and two-line Strong Heterosis Hybrid rice combinations through innovative fpvd analysis method. It was found that the three-line and two-line strong heterosis combinations had different heterosis loci, 98 gene loci were found in the three-line combination and 36 gene loci were found in the two-line combination.

Further, the study also detected that there was a 408kb long genomic fragment deletion on chromosome 7 of Honglian male sterile lines Luohong 3a and Luohong 4a, which can be used to reduce cadmium pollution in rice grains and is of great significance to cultivate new low cadmium rice varieties.